Review



dam dcm electrocompetent e coli  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    New England Biolabs dam dcm electrocompetent e coli
    ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( <t>E.</t> <t>coli</t> ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.
    Dam Dcm Electrocompetent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dam+dcm+electrocompetent+e+coli/dam-%2Fdcm-+Competent+E%2E+coli/bio_rxiv__2025__11__18__689155-166-6-10
    Average 96 stars, based on 293 article reviews
    dam dcm electrocompetent e coli - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes"

    Article Title: Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes

    Journal: bioRxiv

    doi: 10.1101/2025.11.18.689155

    ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( E. coli ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.
    Figure Legend Snippet: ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( E. coli ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.

    Techniques Used: Transformation Assay, Bacteria, Electroporation, Comparison, Selection, Plasmid Preparation

    Related Articles

    Plasmid Preparation:

    Article Title: Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes
    Article Snippet: The final plasmid, pAKgfp1-kan, was transformed into NEB 5-alpha electrocompetent E. coli (New England Biolabs), extracted by plasmid miniprep using the QIAprep Spin Miniprep Kit (Qiagen), and sequence verified. .. The plasmid was then transformed into dam–/dcm– electrocompetent E. coli (New England Biolabs) according to the manufacturer’s instructions, extracted and sequence verified by Eton Bioscience (Boston, MA). .. Individual pGL2 plasmids ( Supplementary Table 1 ) were stored in TransforMax EC100D pir-116 cells (Biosearch Technologies); pAKgfp1-kan was stored in dam–/dcm– E. coli (New England Biolabs).

    Transformation Assay:

    Article Title: Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes
    Article Snippet: The final plasmid, pAKgfp1-kan, was transformed into NEB 5-alpha electrocompetent E. coli (New England Biolabs), extracted by plasmid miniprep using the QIAprep Spin Miniprep Kit (Qiagen), and sequence verified. .. The plasmid was then transformed into dam–/dcm– electrocompetent E. coli (New England Biolabs) according to the manufacturer’s instructions, extracted and sequence verified by Eton Bioscience (Boston, MA). .. Individual pGL2 plasmids ( Supplementary Table 1 ) were stored in TransforMax EC100D pir-116 cells (Biosearch Technologies); pAKgfp1-kan was stored in dam–/dcm– E. coli (New England Biolabs).

    Sequencing:

    Article Title: Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes
    Article Snippet: The final plasmid, pAKgfp1-kan, was transformed into NEB 5-alpha electrocompetent E. coli (New England Biolabs), extracted by plasmid miniprep using the QIAprep Spin Miniprep Kit (Qiagen), and sequence verified. .. The plasmid was then transformed into dam–/dcm– electrocompetent E. coli (New England Biolabs) according to the manufacturer’s instructions, extracted and sequence verified by Eton Bioscience (Boston, MA). .. Individual pGL2 plasmids ( Supplementary Table 1 ) were stored in TransforMax EC100D pir-116 cells (Biosearch Technologies); pAKgfp1-kan was stored in dam–/dcm– E. coli (New England Biolabs).



    Similar Products

    dam dcm electrocompetent e coli  (New England Biolabs)
    96
    New England Biolabs dam dcm electrocompetent e coli
    ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( <t>E.</t> <t>coli</t> ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.
    Dam Dcm Electrocompetent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dam+dcm+electrocompetent+e+coli/dam-%2Fdcm-+Competent+E%2E+coli/bio_rxiv__2025__11__18__689155-166-6-10
    Average 96 stars, based on 1 article reviews
    dam dcm electrocompetent e coli - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    electrocompetent c2925  (New England Biolabs)
    96
    New England Biolabs electrocompetent c2925
    ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( <t>E.</t> <t>coli</t> ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.
    Electrocompetent C2925, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dam+dcm+electrocompetent+e+coli/dam-%2Fdcm-+Comp+E%2Ecoli/us11091782-153-80-83
    Average 96 stars, based on 1 article reviews
    electrocompetent c2925 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( E. coli ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.

    Journal: bioRxiv

    Article Title: Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes

    doi: 10.1101/2025.11.18.689155

    Figure Lengend Snippet: ( A-C ) Effect of buffer, voltage, and waveform on transformation efficiency (TE) in three Gram-negative bacteria. Data are the average of two biological replicates. ( A ) TE using four buffers: water, 25% sorbitol, 10% or 15% glycerol, and 25% sucrose. Data shown was electroporated at 3 kV using exponential decay (see additional voltages in Supplementary Figure 3). ( B ) TE using a range of electroporation voltages: 0.5-3 kV. Cells were washed with 10% glycerol ( E. coli ), 25% sorbitol ( S. amazonensis ), and 15% glycerol ( P. alcaliphila ), and electroporated using exponential decay waveform. Error bars represent standard error. ( C ) Comparison of TE using square or exponential decay waveforms. Data are results for all voltages and buffers tested in panel B. ( D ) Final parameter selection for 24-condition electroporation screen including four buffers, three voltages and two waveforms. ( E-F ) TE following the 24-condition electroporation screen performed on seven bacteria with a single plasmid using ( E ) exponential decay (EX) and ( F ) square (SQ) waveforms. Data are the average of two biological replicates, except P. sakaiensis and C. necator which are a single replicate.

    Article Snippet: The plasmid was then transformed into dam–/dcm– electrocompetent E. coli (New England Biolabs) according to the manufacturer’s instructions, extracted and sequence verified by Eton Bioscience (Boston, MA).

    Techniques: Transformation Assay, Bacteria, Electroporation, Comparison, Selection, Plasmid Preparation